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The superstar musician accepted the Grammy for best pop duo/group performance for "Shallow," which she recorded with her "A Star Is Born" co-star and director, Bradley Cooper.
"I gotta thank God. Thank you for looking out for me, thank you for my family at home; I love you," the 32-year-old performer said as she fought back tears.
"If I don't get another chance to say this — I just want to say I'm so proud to be a part of a movie that addresses mental health issues. They're so important," she continued. "And a lot of artists deal with that, and we got to take care of each other."
"So if you see somebody that's hurting, don't look away," she urged.
Gaga then pleaded with her colleagues and fans to be sure to take care of themselves as well.
"If you're hurting, even though it might be hard, try to find that bravery within yourself to dive deep and go tell somebody and take them up in your head with you," she said, adding, "I love you."
The singer and actress also expressed her gratitude for Cooper, who's in the United Kingdom attending the BAFTA Awards.
"I wish Bradley were with me right now ... I know he wants to be here," Gaga shared, before gushing, "Bradley, I loved singing this song with you."
Gaga and Cooper beat out Christina Aguilera and Demi Lovato for "Fall in Line," Backstreet Boys for "Don’t Go Breaking My Heart," Tony Bennett and Diana Krall for “S Wonderful,” and Maroon 5 and Cardi B for "Girls Like You."
The singer also won two Grammys earlier in the day: best song for visual media for "Shallow" and best pop vocal performance for "Joanne."
Later in the evening, Gaga took to the stage to perform "Shallow" along with her co-songwriters, musicians Mark Ronson, Anthony Rossomando and Andrew Wyatt.
Decked out in a low-cut sequined bodysuit, Gaga prowled around the stage like a glam rocker, shaking her hair wildly and bending down low with the microphone stand, to deliver one of the night's most exciting performances.